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integrin α1 fb12 antibody  (Millipore)


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    Structured Review

    Millipore integrin α1 fb12 antibody
    Integrin α1 Fb12 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B11+(fb12/integrin+%CE%B11+antibody/pm39136993-235-11-14
    Average 90 stars, based on 1 article reviews
    integrin α1 fb12 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Hyperelastic, shape‐memorable, and ultra‐cell‐adhesive degradable polycaprolactone‐polyurethane copolymer for tissue regeneration
    Article Snippet: ISB‐P films were punched with a 6 mm diameter and sterilized by EO gas. hMSCs (2 × 10 4 cells/100 μl) were preincubated with 10 μg/ml of function‐blocking antibodies against integrin subunits α1 (FB12), α2 (P1E6), α3 (P4C2), α4 (P4C2), α5 (P1D6), α6 (NKI‐GOH3), β1 (6S6), β2 (CD18), and β3 (25E11; Millipore, Bedford, MA) in alpha MEM separately for 15 min at 37°C in a humidified atmosphere of 5% CO 2 .

    Article Title: Peptide for promoting bone formation or inhibiting bone resorption and use thereof
    Article Snippet: Cells (5×104 cells/250 μL) were preincubated with 5 mM EDTA, 500 μM MnCl2, 100 μg/mL heparin, and 10 μg/mL function-blocking antibodies against integrin α1 (FB12), α2 (P1E6), α3 (P1B5), α4 (P4C2), α5 (P1D6), α6 (NKI-GoH3), β3 (B3A; Chemicon), av (AV1), β1 (6S6; Millipore), or β3 (B3A) subunits at 37° C. for 15 minutes.

    Article Title: Hyperelastic, shape-memorable, and ultra-cell-adhesive degradable polycaprolactone-polyurethane copolymer for tissue regeneration.
    Article Snippet: ISB-P films were punched with a 6 mm diameter and sterilized by EO gas. hMSCs (2 104 cells/100 μl) were preincubated with 10 μg/ml of function-blocking antibodies against integrin subunits α1 (FB12), α2 (P1E6), α3 (P4C2), α4 (P4C2), α5 (P1D6), α6 (NKI-GOH3), β1 (6S6), β2 (CD18), and β3 (25E11; Millipore, Bedford, MA) in alpha MEM separately for 15 min at 37 C in a humidified atmosphere of 5% CO2.

    Article Title: Fibroblast integrin α11β1 is a collagen assembly receptor in mechanoregulated fibrillar adhesions.
    Article Snippet: The primary antibodies and the dilutions used are listed as follows: mouse anti-human integrin α11 monoclonal antibody (mAb) 203E3 [49] (1.25μg/mL), rabbit anti-integrin α2 mAb (1:300, EPR5788, Abcam, Cat# ab133557), mouse anti-human integrin α2 mAb (1:200, P1E6, Millipore, Cat# MAB1950Z), rabbit anti-integrin α1/CD49a mAb (1:400, E5D6Q, Cell signaling, Cat#87841S), mouse anti-integrin α1/CD49a mAb (1:100, FB12, Sigma-Aldrich, Cat#MAB1973Z), rabbit anti-tensin-1 pAb (1:400, Atlas Antibodies, Cat# HPA036089), rabbit anti-tensin-3 pAb (1:400, Atlas Antibodies, Cat# HPA055338), mouse anti-human α5 mAb (1:200 SNAKA51, Merck, Cat# MABT201), mouse anti-human integrin 1 mAb (1:400, HUTS-4, Millipore, Cat# MAB2079-AF555), rabbit anti-FAKY397 (1:1000, Cell Signaling, Cat# 8556S), mouse anti-EDA fibronectin (1:400, Novus Biologicals, Cat# NB600-946) and rabbit anti-collagen I pAb (1:400, Gentaur, Cat# OARA02579).

    Article Title: Integrin α2β1 regulates collagen I tethering to modulate hyperresponsiveness in reactive airway disease models
    Article Snippet: Integrin antibodies used were monoclonal anti–integrin α1 (FB12, MilliporeSigma), α2 (P1E6, MilliporeSigma and HMa2, Invitrogen), α3 (P1B5, BioLegend), α6 (NKI-GoH3, MilliporeSigma), α7 (6A11, MBL) (50, 51), α11 (203E3, a gift from Donald Gullberg at the University of Bergen, Norway) (52), and activated β1 (HUTS-4, MilliporeSigma).

    Centrifugation:

    Article Title: Evaluation of late outgrowth endothelial progenitor cell and umbilical vein endothelial cell responses to thromboresistant collagen-mimetic hydrogels.
    Article Snippet: Briefly, EOCs or HUVECs were resuspended at 200,000 cells per mL, placed on ice, and exposed to 2 μM Calcein AM (Life Technologies) in staining buffer (1% BSA in PBS) for 30 min. .. The cells were then pelleted by centrifugation and washed twice with staining buffer, after which they were resuspended in 100 μL staining buffer containing 10 μg/mL of appropriate primary antibody (integrin α1: clone FB12, integrin α2: clone P1E6; Millipore), or IgG control. ..

    Staining:

    Article Title: Evaluation of late outgrowth endothelial progenitor cell and umbilical vein endothelial cell responses to thromboresistant collagen-mimetic hydrogels.
    Article Snippet: Briefly, EOCs or HUVECs were resuspended at 200,000 cells per mL, placed on ice, and exposed to 2 μM Calcein AM (Life Technologies) in staining buffer (1% BSA in PBS) for 30 min. .. The cells were then pelleted by centrifugation and washed twice with staining buffer, after which they were resuspended in 100 μL staining buffer containing 10 μg/mL of appropriate primary antibody (integrin α1: clone FB12, integrin α2: clone P1E6; Millipore), or IgG control. ..

    Control:

    Article Title: Evaluation of late outgrowth endothelial progenitor cell and umbilical vein endothelial cell responses to thromboresistant collagen-mimetic hydrogels.
    Article Snippet: Briefly, EOCs or HUVECs were resuspended at 200,000 cells per mL, placed on ice, and exposed to 2 μM Calcein AM (Life Technologies) in staining buffer (1% BSA in PBS) for 30 min. .. The cells were then pelleted by centrifugation and washed twice with staining buffer, after which they were resuspended in 100 μL staining buffer containing 10 μg/mL of appropriate primary antibody (integrin α1: clone FB12, integrin α2: clone P1E6; Millipore), or IgG control. ..



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    Identification of cell surface receptors binding to human laminin <t>α1-5</t> LG45 modules. ( A ) Adhesion of HEK293 (WT), FKTN -knockout HEK293 (KO), and FKTN -rescued KO cells (Res/KO) to laminin α1 and α2 LG45 modules. The KO cells are lacking the glycosylation on α-DG associated with laminin binding. The attached cells were stained and counted under a microscope. ( B ) Inhibitory effects of antibodies to <t>integrin</t> α6 (ITGA6) and β1 (ITGB1) on cell adhesion in the presence of heparin. HEK293 cells pre-incubated with antibodies and heparin were added to the recombinant protein-coated wells. The attached cells were observed by microscopy.
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    Expression of trophoblast markers and markers for trophoblast subpopulations in primary first trimester trophoblasts, ACH-3P and AC1-1. The expression of the trophoblast marker cytokeratin-7 (CK7) was determined by immunocytochemistry and of markers for trophoblast subtypes (HLA-G, integrins) were detected by FACS analysis. Secretion of hCG was measured after 24 hours in the culture supernatant and normalized to the cell number. The activity of MMP2 and MMP9 was measured by gelatin gel zymography. + indicates ~20–40% of cells. ++ indicates ~40–90%; +++ indicates 100% of cells; – indicates no expression.
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    Collagen gel stimulated migration of human amnion cells. ( A and B ) Wound healing scratch assay of primary human amnion mesenchymal cells with collagen gel. ( A ) Representative images at 0 and 72 h. Bars, 200 µm. ( B ) Percent closure of scratched area of amnion mesenchymal cells treated with different concentration of collagen gel. ( C ) Wound scratch assay with <t>integrin</t> blocking antibodies. Scratched amnion mesenchymal cells were covered by collagen gel including 10 μg/ml of <t>α1,</t> α2, or β1 integrin blocking antibodies or control IgG. After solidification of gel, serum free medium containing same concentration (10 μg/ml) of blocking antibodies or control IgG was overlaid. ( D ) Wound scratch assay with inhibitors of α1β1 and α1β2 integrin, DDR1, and DDR2. Scratched cells were treated with collagen gel including 1 μM inhibitor (α1β1, α2β1 integrin, DDR1, DDR2) or vehicle (DMSO or water). Serum free medium including same concentration (1 μM) of inhibitors were overlaid. ( E ) DDR1 and DDR2 mRNA expression in human amnion epithelial and mesenchymal cells. Gene expression was normalized to that of GAPDH. The value of each gene was compared by including cDNA standard samples in the same assay plate (standard curve method). Error bars represent SD. n = 3 in each group. * P < 0.05, ** P < 0.01.
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    Image Search Results


    Identification of cell surface receptors binding to human laminin α1-5 LG45 modules. ( A ) Adhesion of HEK293 (WT), FKTN -knockout HEK293 (KO), and FKTN -rescued KO cells (Res/KO) to laminin α1 and α2 LG45 modules. The KO cells are lacking the glycosylation on α-DG associated with laminin binding. The attached cells were stained and counted under a microscope. ( B ) Inhibitory effects of antibodies to integrin α6 (ITGA6) and β1 (ITGB1) on cell adhesion in the presence of heparin. HEK293 cells pre-incubated with antibodies and heparin were added to the recombinant protein-coated wells. The attached cells were observed by microscopy.

    Journal: Scientific Reports

    Article Title: Chain-specificity of laminin α1-5 LG45 modules in the recognition of carbohydrate-linked receptors and intramolecular binding

    doi: 10.1038/s41598-023-37533-y

    Figure Lengend Snippet: Identification of cell surface receptors binding to human laminin α1-5 LG45 modules. ( A ) Adhesion of HEK293 (WT), FKTN -knockout HEK293 (KO), and FKTN -rescued KO cells (Res/KO) to laminin α1 and α2 LG45 modules. The KO cells are lacking the glycosylation on α-DG associated with laminin binding. The attached cells were stained and counted under a microscope. ( B ) Inhibitory effects of antibodies to integrin α6 (ITGA6) and β1 (ITGB1) on cell adhesion in the presence of heparin. HEK293 cells pre-incubated with antibodies and heparin were added to the recombinant protein-coated wells. The attached cells were observed by microscopy.

    Article Snippet: Anti-integrin α1 (FB12) and anti-α-DG (3D7 and IIH6) antibodies were purchased from Merck (Kenilworth, NJ, USA).

    Techniques: Binding Assay, Knock-Out, Staining, Microscopy, Incubation, Recombinant

    Summary of the binding properties of αLG45 modules.

    Journal: Scientific Reports

    Article Title: Chain-specificity of laminin α1-5 LG45 modules in the recognition of carbohydrate-linked receptors and intramolecular binding

    doi: 10.1038/s41598-023-37533-y

    Figure Lengend Snippet: Summary of the binding properties of αLG45 modules.

    Article Snippet: Anti-integrin α1 (FB12) and anti-α-DG (3D7 and IIH6) antibodies were purchased from Merck (Kenilworth, NJ, USA).

    Techniques: Binding Assay

    Expression of trophoblast markers and markers for trophoblast subpopulations in primary first trimester trophoblasts, ACH-3P and AC1-1. The expression of the trophoblast marker cytokeratin-7 (CK7) was determined by immunocytochemistry and of markers for trophoblast subtypes (HLA-G, integrins) were detected by FACS analysis. Secretion of hCG was measured after 24 hours in the culture supernatant and normalized to the cell number. The activity of MMP2 and MMP9 was measured by gelatin gel zymography. + indicates ~20–40% of cells. ++ indicates ~40–90%; +++ indicates 100% of cells; – indicates no expression.

    Journal: BMC Developmental Biology

    Article Title: The first trimester human trophoblast cell line ACH-3P: A novel tool to study autocrine/paracrine regulatory loops of human trophoblast subpopulations – TNF-α stimulates MMP15 expression

    doi: 10.1186/1471-213X-7-137

    Figure Lengend Snippet: Expression of trophoblast markers and markers for trophoblast subpopulations in primary first trimester trophoblasts, ACH-3P and AC1-1. The expression of the trophoblast marker cytokeratin-7 (CK7) was determined by immunocytochemistry and of markers for trophoblast subtypes (HLA-G, integrins) were detected by FACS analysis. Secretion of hCG was measured after 24 hours in the culture supernatant and normalized to the cell number. The activity of MMP2 and MMP9 was measured by gelatin gel zymography. + indicates ~20–40% of cells. ++ indicates ~40–90%; +++ indicates 100% of cells; – indicates no expression.

    Article Snippet: For staining of membrane antigens on living cells the following antibodies were used: anti-integrin α1 (Clone FB12; 1:500; Chemicon), anti-integrin α6 (Clone GoH3; 1:500; BD Pharmingen), anti-integrin β4 (BD Pharmingen; 1:500), anti-integrin α5β1 (Dako; 1:25), and HLA-G (Clone MEM-G/9; 1:500; Abcam).

    Techniques: Expressing, Marker, Immunocytochemistry, Activity Assay, Zymography

    Antibodies and their appropriate IgG controls used for FACS analysis.

    Journal: BMC Developmental Biology

    Article Title: The first trimester human trophoblast cell line ACH-3P: A novel tool to study autocrine/paracrine regulatory loops of human trophoblast subpopulations – TNF-α stimulates MMP15 expression

    doi: 10.1186/1471-213X-7-137

    Figure Lengend Snippet: Antibodies and their appropriate IgG controls used for FACS analysis.

    Article Snippet: For staining of membrane antigens on living cells the following antibodies were used: anti-integrin α1 (Clone FB12; 1:500; Chemicon), anti-integrin α6 (Clone GoH3; 1:500; BD Pharmingen), anti-integrin β4 (BD Pharmingen; 1:500), anti-integrin α5β1 (Dako; 1:25), and HLA-G (Clone MEM-G/9; 1:500; Abcam).

    Techniques:

    Antibodies Used in the Study.

    Journal: International Journal of Molecular Sciences

    Article Title: Chondroitin Sulfate Promotes the Proliferation of Keloid Fibroblasts Through Activation of the Integrin and Protein Kinase B Pathways

    doi: 10.3390/ijms21061955

    Figure Lengend Snippet: Antibodies Used in the Study.

    Article Snippet: integrin α1 , Mouse monoclonal , IgG1 , FB12 , 1:500 , MAB1973Z , Merch Millipore , C.

    Techniques: Western Blot, Proliferation Assay

    Key Resources Table

    Journal: Structure (London, England : 1993)

    Article Title: Magnesium Activates Microsecond Dynamics to Regulate Integrin-Collagen Recognition

    doi: 10.1016/j.str.2018.05.010

    Figure Lengend Snippet: Key Resources Table

    Article Snippet: Mouse anti-Integrin α1 antibody, clone FB12 , Millipore EMD , Cat#MAB1973; RRID: AB_2129087.

    Techniques: Virus, Recombinant, Dispersion, Plasmid Preparation, Software

    Collagen gel stimulated migration of human amnion cells. ( A and B ) Wound healing scratch assay of primary human amnion mesenchymal cells with collagen gel. ( A ) Representative images at 0 and 72 h. Bars, 200 µm. ( B ) Percent closure of scratched area of amnion mesenchymal cells treated with different concentration of collagen gel. ( C ) Wound scratch assay with integrin blocking antibodies. Scratched amnion mesenchymal cells were covered by collagen gel including 10 μg/ml of α1, α2, or β1 integrin blocking antibodies or control IgG. After solidification of gel, serum free medium containing same concentration (10 μg/ml) of blocking antibodies or control IgG was overlaid. ( D ) Wound scratch assay with inhibitors of α1β1 and α1β2 integrin, DDR1, and DDR2. Scratched cells were treated with collagen gel including 1 μM inhibitor (α1β1, α2β1 integrin, DDR1, DDR2) or vehicle (DMSO or water). Serum free medium including same concentration (1 μM) of inhibitors were overlaid. ( E ) DDR1 and DDR2 mRNA expression in human amnion epithelial and mesenchymal cells. Gene expression was normalized to that of GAPDH. The value of each gene was compared by including cDNA standard samples in the same assay plate (standard curve method). Error bars represent SD. n = 3 in each group. * P < 0.05, ** P < 0.01.

    Journal: Scientific Reports

    Article Title: Collagen Type 1 Accelerates Healing of Ruptured Fetal Membranes

    doi: 10.1038/s41598-017-18787-9

    Figure Lengend Snippet: Collagen gel stimulated migration of human amnion cells. ( A and B ) Wound healing scratch assay of primary human amnion mesenchymal cells with collagen gel. ( A ) Representative images at 0 and 72 h. Bars, 200 µm. ( B ) Percent closure of scratched area of amnion mesenchymal cells treated with different concentration of collagen gel. ( C ) Wound scratch assay with integrin blocking antibodies. Scratched amnion mesenchymal cells were covered by collagen gel including 10 μg/ml of α1, α2, or β1 integrin blocking antibodies or control IgG. After solidification of gel, serum free medium containing same concentration (10 μg/ml) of blocking antibodies or control IgG was overlaid. ( D ) Wound scratch assay with inhibitors of α1β1 and α1β2 integrin, DDR1, and DDR2. Scratched cells were treated with collagen gel including 1 μM inhibitor (α1β1, α2β1 integrin, DDR1, DDR2) or vehicle (DMSO or water). Serum free medium including same concentration (1 μM) of inhibitors were overlaid. ( E ) DDR1 and DDR2 mRNA expression in human amnion epithelial and mesenchymal cells. Gene expression was normalized to that of GAPDH. The value of each gene was compared by including cDNA standard samples in the same assay plate (standard curve method). Error bars represent SD. n = 3 in each group. * P < 0.05, ** P < 0.01.

    Article Snippet: Blocking antibodies or inhibitors were used as follows: anti-integrin α1 antibody (clone FB12, #MAB1973Z, Millipore), anti-integrin α2 antibody (clone P1E6, #MAB1950Z, Millipore), anti-integrin β1 antibody (clone 6S6, #MAB2253Z, Millipore), Mouse IgG1 control antibody (Clone 107.3, #554721, BD Bioscience), DDR1 inhibitor (DDR1-IN-1, #5077, Tocris), DDR2 inhibitor (LCB 03-0110, #5592, Tocris), integrin α1β1 inhibitor (Obtustatin, #4664, Tocris) and integrin α2β1 inhibitor (BTT 3033, #4724, Tocris).

    Techniques: Migration, Wound Healing Assay, Concentration Assay, Blocking Assay, Control, Expressing, Gene Expression